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creb inhibitor  (MedChemExpress)


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    Structured Review

    MedChemExpress creb inhibitor
    a cAMP quantitation of keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 2 biologically independent samples for fibroblasts treated with epinephrine in the presence of metoprolol, and n = 3 biologically independent samples for other groups). b qPCR analysis of TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence <t>of</t> <t>PKA</t> inhibitor or <t>CREB</t> inhibitor for 6 h ( n = 4 biologically independent samples for fibroblasts treated with epinephrine in the presence of CREB inhibitor, and n = 5 biologically independent samples for other groups). c Immunoblotting and quantitative analysis of IBSP and TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 24 h ( n = 3 biologically independent samples). d qPCR analysis of the abundance of the TSN promoter that immunoprecipitated with αCREB1 or IgG control in keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 6 biologically independent samples for Epinephrine-CREB1 and Epinephrine & Metoprolol-CREB1, and n = 5 biologically independent samples for other groups). Data are presented as the percentages of αCREB1- or IgG-pulled TSN promoter relative to that in the input. Data represent the mean ± SD; p -values were determined by one-way ANOVA. Data are representative of at least 2 independent experiments.
    Creb Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/creb+inhibitor/666-15/pmc13376770-310-26-29
    Average 95 stars, based on 93 article reviews
    creb inhibitor - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Catecholaminergic neurons boost fibroblast osteogenic activity in keloid"

    Article Title: Catecholaminergic neurons boost fibroblast osteogenic activity in keloid

    Journal: Nature Communications

    doi: 10.1038/s41467-026-72823-9

    a cAMP quantitation of keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 2 biologically independent samples for fibroblasts treated with epinephrine in the presence of metoprolol, and n = 3 biologically independent samples for other groups). b qPCR analysis of TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 6 h ( n = 4 biologically independent samples for fibroblasts treated with epinephrine in the presence of CREB inhibitor, and n = 5 biologically independent samples for other groups). c Immunoblotting and quantitative analysis of IBSP and TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 24 h ( n = 3 biologically independent samples). d qPCR analysis of the abundance of the TSN promoter that immunoprecipitated with αCREB1 or IgG control in keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 6 biologically independent samples for Epinephrine-CREB1 and Epinephrine & Metoprolol-CREB1, and n = 5 biologically independent samples for other groups). Data are presented as the percentages of αCREB1- or IgG-pulled TSN promoter relative to that in the input. Data represent the mean ± SD; p -values were determined by one-way ANOVA. Data are representative of at least 2 independent experiments.
    Figure Legend Snippet: a cAMP quantitation of keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 2 biologically independent samples for fibroblasts treated with epinephrine in the presence of metoprolol, and n = 3 biologically independent samples for other groups). b qPCR analysis of TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 6 h ( n = 4 biologically independent samples for fibroblasts treated with epinephrine in the presence of CREB inhibitor, and n = 5 biologically independent samples for other groups). c Immunoblotting and quantitative analysis of IBSP and TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 24 h ( n = 3 biologically independent samples). d qPCR analysis of the abundance of the TSN promoter that immunoprecipitated with αCREB1 or IgG control in keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 6 biologically independent samples for Epinephrine-CREB1 and Epinephrine & Metoprolol-CREB1, and n = 5 biologically independent samples for other groups). Data are presented as the percentages of αCREB1- or IgG-pulled TSN promoter relative to that in the input. Data represent the mean ± SD; p -values were determined by one-way ANOVA. Data are representative of at least 2 independent experiments.

    Techniques Used: Quantitation Assay, Expressing, Western Blot, Immunoprecipitation, Control

    Related Articles

    In Vitro:

    Article Title: The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs
    Article Snippet: .. The in vitro treatment conditions for the small-molecule compound were as follows: GLP1RA semaglutide acetate (10 nM, 24 h), GIPRA (Pro3) GIP (0.5 nM, 24 h), GLP-1R/GIPR agonist-1 (10 nM, 24 h) also termed as 2GRA in this study, BGM0504 (2 nM, 24 h, BrightGene Pharmaceutical Co., Ltd., China) [ ], CREB inhibitor 666-15 (1 μM, 2 h, HY-101120, MCE), Akt inhibitor MK-2206 (5 μM, 24 h, HY-108232, MCE), ERK1/2 inhibitor SCH772984 (0.5 μM, 24 h, HY-50846, MCE), β-catenin inhibitor IN-3 (5 μM, 24 h, HY-147007, MCE), STAT3-IN-14 (5 μM, 2 h, HY-N10472, MCE). .. PDLSCs were induced to differentiate into osteogenic (HUXXC-90021, OriCell, China), adipogenic (HUXXC-90031, OriCell), and chondrogenic (HUXXC-90041, OriCell) lineages using commercial differentiation kits according to the manufacturer’s protocols.

    Article Title: The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs.
    Article Snippet: .. The in vitro treatment conditions for the small-molecule compound were as follows: GLP1RA semaglutide acetate (10 nM, 24 h), GIPRA (Pro3) GIP (0.5 nM, 24 h), GLP1R/GIPR agonist-1 (10 nM, 24 h) also termed as 2GRA in this study, BGM0504 (2 nM, 24 h, BrightGene Pharmaceutical Co., Ltd., China) [13], CREB inhibitor 666-15 (1 μM, 2 h, HY-101120, MCE), Akt inhibitor MK-2206 (5 μM, 24 h, HY-108232, MCE), ERK1/2 inhibitor SCH772984 (0.5 μM, 24 h, HY-50846, MCE), β-catenin inhibitor IN-3 (5 μM, 24 h, HY-147007, MCE), STAT3-IN-14 (5 μM, 2 h, HY-N10472, MCE). .. PDLSCs were induced to differentiate into osteogenic (HUXXC-90021, OriCell, China), adipogenic (HUXXC-90031, OriCell), and chondrogenic (HUXXC-90041, OriCell) lineages using commercial differentiation kits according to the manufacturer’s protocols.

    Virus:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    Recombinant:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    Lysis:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    SDS Page:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    Bicinchoninic Acid Protein Assay:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    Western Blot:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    SYBR Green Assay:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    Real-time Polymerase Chain Reaction:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    Electroporation:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    Hydroxyproline Assay:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    Liposomes:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    RNA Sequencing:

    Article Title: Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis.
    Article Snippet: Article Dectin-1 facilitates lung fungal-mediated pulmonary fibrosis

    other:

    Article Title: Catecholaminergic neurons boost fibroblast osteogenic activity in keloid
    Article Snippet: The cells were washed, blocked with 5% goat serum/1% BSA/PBS for 1 h, and stained with anti-TSN (Absin cat. abs133594, 1:250 dilution) and Phalloidin (Absin cat. abs47048271, 1:200 dilution).

    Injection:

    Article Title: Targeting CREB remodels the immune microenvironment to enhance immunotherapy responses in pancreatic cancer
    Article Snippet: .. Once the tumors were established following initial implantation, mice were randomized to receive intraperitoneal (i.p.) injection of CREB inhibitor (CREBi, 666-15, 20mg/kg) [MedChemExpress, #HY-101120] administered 3x weekly, αPD-1 antibody (BioXCell, Clone #BE0273, 200 μg/mouse, intraperitoneal injection twice weekly), or a combination of CREBi and αPD-1 until moribund for survival studies. .. EC359 [MedChem Express, HY-120142] is a potent inhibitor of leukemia inhibitory factor receptor (LIFR) were administered subcutaneously at a dose of 15 mg/kg, thrice weekly for a total of 2 weeks.



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    a cAMP quantitation of keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 2 biologically independent samples for fibroblasts treated with epinephrine in the presence of metoprolol, and n = 3 biologically independent samples for other groups). b qPCR analysis of TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence <t>of</t> <t>PKA</t> inhibitor or <t>CREB</t> inhibitor for 6 h ( n = 4 biologically independent samples for fibroblasts treated with epinephrine in the presence of CREB inhibitor, and n = 5 biologically independent samples for other groups). c Immunoblotting and quantitative analysis of IBSP and TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 24 h ( n = 3 biologically independent samples). d qPCR analysis of the abundance of the TSN promoter that immunoprecipitated with αCREB1 or IgG control in keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 6 biologically independent samples for Epinephrine-CREB1 and Epinephrine & Metoprolol-CREB1, and n = 5 biologically independent samples for other groups). Data are presented as the percentages of αCREB1- or IgG-pulled TSN promoter relative to that in the input. Data represent the mean ± SD; p -values were determined by one-way ANOVA. Data are representative of at least 2 independent experiments.
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    a cAMP quantitation of keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 2 biologically independent samples for fibroblasts treated with epinephrine in the presence of metoprolol, and n = 3 biologically independent samples for other groups). b qPCR analysis of TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence <t>of</t> <t>PKA</t> inhibitor or <t>CREB</t> inhibitor for 6 h ( n = 4 biologically independent samples for fibroblasts treated with epinephrine in the presence of CREB inhibitor, and n = 5 biologically independent samples for other groups). c Immunoblotting and quantitative analysis of IBSP and TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 24 h ( n = 3 biologically independent samples). d qPCR analysis of the abundance of the TSN promoter that immunoprecipitated with αCREB1 or IgG control in keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 6 biologically independent samples for Epinephrine-CREB1 and Epinephrine & Metoprolol-CREB1, and n = 5 biologically independent samples for other groups). Data are presented as the percentages of αCREB1- or IgG-pulled TSN promoter relative to that in the input. Data represent the mean ± SD; p -values were determined by one-way ANOVA. Data are representative of at least 2 independent experiments.
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    a cAMP quantitation of keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 2 biologically independent samples for fibroblasts treated with epinephrine in the presence of metoprolol, and n = 3 biologically independent samples for other groups). b qPCR analysis of TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence <t>of</t> <t>PKA</t> inhibitor or <t>CREB</t> inhibitor for 6 h ( n = 4 biologically independent samples for fibroblasts treated with epinephrine in the presence of CREB inhibitor, and n = 5 biologically independent samples for other groups). c Immunoblotting and quantitative analysis of IBSP and TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 24 h ( n = 3 biologically independent samples). d qPCR analysis of the abundance of the TSN promoter that immunoprecipitated with αCREB1 or IgG control in keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 6 biologically independent samples for Epinephrine-CREB1 and Epinephrine & Metoprolol-CREB1, and n = 5 biologically independent samples for other groups). Data are presented as the percentages of αCREB1- or IgG-pulled TSN promoter relative to that in the input. Data represent the mean ± SD; p -values were determined by one-way ANOVA. Data are representative of at least 2 independent experiments.
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    Image Search Results


    a cAMP quantitation of keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 2 biologically independent samples for fibroblasts treated with epinephrine in the presence of metoprolol, and n = 3 biologically independent samples for other groups). b qPCR analysis of TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 6 h ( n = 4 biologically independent samples for fibroblasts treated with epinephrine in the presence of CREB inhibitor, and n = 5 biologically independent samples for other groups). c Immunoblotting and quantitative analysis of IBSP and TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 24 h ( n = 3 biologically independent samples). d qPCR analysis of the abundance of the TSN promoter that immunoprecipitated with αCREB1 or IgG control in keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 6 biologically independent samples for Epinephrine-CREB1 and Epinephrine & Metoprolol-CREB1, and n = 5 biologically independent samples for other groups). Data are presented as the percentages of αCREB1- or IgG-pulled TSN promoter relative to that in the input. Data represent the mean ± SD; p -values were determined by one-way ANOVA. Data are representative of at least 2 independent experiments.

    Journal: Nature Communications

    Article Title: Catecholaminergic neurons boost fibroblast osteogenic activity in keloid

    doi: 10.1038/s41467-026-72823-9

    Figure Lengend Snippet: a cAMP quantitation of keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 2 biologically independent samples for fibroblasts treated with epinephrine in the presence of metoprolol, and n = 3 biologically independent samples for other groups). b qPCR analysis of TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 6 h ( n = 4 biologically independent samples for fibroblasts treated with epinephrine in the presence of CREB inhibitor, and n = 5 biologically independent samples for other groups). c Immunoblotting and quantitative analysis of IBSP and TSN expression in keloid fibroblasts treated or not with epinephrine in the presence or absence of PKA inhibitor or CREB inhibitor for 24 h ( n = 3 biologically independent samples). d qPCR analysis of the abundance of the TSN promoter that immunoprecipitated with αCREB1 or IgG control in keloid fibroblasts treated or not with epinephrine in the presence or absence of metoprolol for 30 min ( n = 6 biologically independent samples for Epinephrine-CREB1 and Epinephrine & Metoprolol-CREB1, and n = 5 biologically independent samples for other groups). Data are presented as the percentages of αCREB1- or IgG-pulled TSN promoter relative to that in the input. Data represent the mean ± SD; p -values were determined by one-way ANOVA. Data are representative of at least 2 independent experiments.

    Article Snippet: For the assays involving PKA or CREB antagonism, keloid fibroblasts were treated with 1 μM PKA Inhibitor Fragment (6-22) amide TFA (MCE cat. HY-P1290A) or the CREB inhibitor 666-15 (MCE cat. HY-101120) for 1 h before the addition of epinephrine.

    Techniques: Quantitation Assay, Expressing, Western Blot, Immunoprecipitation, Control